Multiplexing and Spatial Equipment
The facility has been at the forefront of multiplexing since the beginning of 2022 with the first Cell DIVE, a purpose build multiplexing slide scanner, outside the US. The technology along with our other spatial multiplexing platform (PhenoCycler Fusion 2) allows tissue to be interrogated in cyclic rounds of staining and destaining building up a large panel of protein markers. Where traditionally researchers have been limited to looking at 3-8 markers we are now able to look at anywhere from 4-100 markers on a single slide.
Multiplex Equipment
About
The Cell DIVE is capable of imaging 60+ fluorescent antibody markers in sequential rounds of staining and is the first to be installed in academia outside of the US. The system is compatible with commercially available antibodies enabling the generation of multiplexed maps of proteins at the single cell level whilst maintaining tissue/sample architecture in a high-throughput manner.
Key Features
Purpose-built imager with user-friendly software that gives pixel perfect image registration and provides flexible and scalable throughput. Integrated with robotic slide handing, imaging can be conducted 24/7 and includes sample tracking software.
Single cell morphological analysis (including Spatial) is possible using industry gold standard HALO software on high performance workstations. The equipment is fully supported by dedicated facility staff and is open for use by all QMUL staff. For more information, please contact the facility manager Dr Luke Gammon (l.gammon@qmul.ac.uk). We’re here to help with costings and preliminary data for grant applications!
About
The PhenoCycler Fusion 2 is a ultra high plex slide scanner capable of imaging 100+ fluorescent antibody markers with automated sequential rounds of staining.
Key Features
With off-the-shelf panels (up to 60plex) available the hard work of panel design in minimised. Using oligonucleotide Barcode Technology each antibody is tagged with a unique oligonucleotide barcode, enabling PhenoCycler-Fusion 2.0 to seamlessly scale the number of biomarkers identified and quantified across the same tissue section.
About
The BOND RXm is an automate staining platform which can be tailored to the users protocol steps.
Key Features
The system is capable of handling up to 30 slides and perform the following tasks:
- Dewaxing
- Antigen retrieval
- Staining (IHC, IF)
About
The BAB platform works seamlessly with the Cell DIVE ClickWell slide holders to automate staining.
Key Features
The system is capable of handling up to 15 ClickWells and perform the following tasks:
- Slide washing
- Dye-inactivation
- Staining
About
The Biocare decloaking chamber allows for precision control of temperature and pressure for antigen retrieval.
Taking up to 72 slides for a single pH or 24 slides for a two pH treatments.